DNA digestion by Nuclease

Dynamic Imaging of the DNA digestion process
(DNA : pUC18 plasmid DNA , DNA digestion: Bal31 nuclease)


Scan speed: 1frame/sec. (30 x)

Movie is the observation of activity of Bal31 and it move along DNA and gradually digested from the ends of DNA chain. Finally, DNA molecules were digested completely, and circular DNA was not digested. High light dots were Bal31 molecules and they bind to the various positions of DNA .


DNA digestion by Nuclease